Application
Western blot
Sample
Pig Cell lysate - whole cell (heart)
Gel Running Conditions
Reduced Denaturing
Loading amount
5 µg
Specification
heart
Blocking step
Licor as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 24°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500
Secondary antibody
Dilution
1/15000
Name
Non-Abcam antibody was used: IRDye 800CW Donkey anti-Rabbit IgG
Host species: Rabbit
Clonality: Polyclonal
Conjugation: IRDye 800CW
Host species: Rabbit
Clonality: Polyclonal
Conjugation: IRDye 800CW
Detection
Detection method
Licor imaging
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 141 kDa Non-specific: 100 kDa
Additional data
Abcam response
We would like to thank this researcher for submitting their review. This antibody had not been tested for cross reactivity with pig and we were unsure of what the results would be.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Nov 04 2016