Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing
Sample
Rat Cell lysate - whole cell (Primary aleveolar epithelial cells)
Specification
Primary aleveolar epithelial cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST 0.1%
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit IgG - HRP :sc
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL+
Bands
Specific: 100 kDa Non-specific: 130, 90 kDa
Exposure
5 minute(s) and 0 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 May 15 2014