概述

  • 产品名称
  • 描述
    兔多克隆抗体to DGKE
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide (Human) derived from an internal region of DGKE

  • 阳性对照
    • Extracts from K562 cells.

性能

应用

Our Abpromise guarantee covers the use of ab69760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).

靶标

  • 功能
    Highly selective for arachidonate-containing species of diacylglycerol (DAG). May terminate signals transmitted through arachidonoyl-DAG or may contribute to the synthesis of phospholipids with defined fatty acid composition.
  • 组织特异性
    Expressed predominantly in testis.
  • 序列相似性
    Belongs to the eukaryotic diacylglycerol kinase family.
    Contains 1 DAGKc domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AHUS7 antibody
    • DAG kinase epsilon antibody
    • DAGK 5 antibody
    • DAGK 6 antibody
    • DAGK5 antibody
    • DAGK6 antibody
    • DGK antibody
    • DGK epsilon antibody
    • DGK-epsilon antibody
    • DGKE antibody
    • DGKE_HUMAN antibody
    • Diacylglycerol kinase epsilon 64kDa antibody
    • Diacylglycerol kinase epsilon antibody
    • Diglyceride kinase epsilon antibody
    • NPHS7 antibody
    see all

图片

  • All lanes : Anti-DGKE antibody (ab69760) at 1/500 dilution

    Lane 1 : Extracts from K562 cells
    Lane 2 : Extracts from K562 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 64 kDa
    Observed band size : 64 kDa
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

实验方案

文献

This product has been referenced in:

See 1 Publication for this product

客户评价及客户问答

The blocking peptide for ab69760 anti DGKE antibody is now listed on our catalog. The product id is ab156171 DGKE peptide. The product page may be found at: http://www.abcam.com/index.html?datasheet=156171

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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