Application
Western blot
Sample
Mouse Recombinant protein (lysate of Cos'7 overexpressing GFP-human, mouse,)
Loading amount
75 µg
Specification
lysate of Cos'7 overexpressing GFP-human, mouse,
Gel Running Conditions
Reduced Denaturing (7.5%)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Dilution
1/500
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS incluiding 0.3% trinton
Secondary antibody
Name
Non-Abcam antibody was used: anit-mouse IgG (Fab faragment)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Exposure
15 minute(s) and 0 second(s)
Positive control
human DGKalpha and Pig DGKalpha
Additional data
Additional Notes
The antibody is very nice to detect human DGKa and pig one but can not recoginize mouse one.
Abcam response
Thank you for your review of this untested species. Your data is valuable to us and our customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Y Shirai
Verified customer
提交于 Aug 05 2011