Application
Western blot
Sample
Mouse Cell lysate - whole cell (Hepa1-6 hepatoma cells)
Loading amount
40 µg
Specification
Hepa1-6 hepatoma cells
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-goat IgG-HRP
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 45 kDa Non-specific: 47, 50, 52, 55 kDa
Positive control
Liver cell lysate
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Apr 17 2012