Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (E12.5 Male Gonad)
Specification
E12.5 Male Gonad
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Other product details
Dilution
1/100
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS/BSA/Triton
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti mouse Alexa 568
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor® 568
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor® 568
Dilution
1/300
Additional data
Additional Notes
Sections were fixed 4 hours, 4% PFA anti-DDX4 antibody (red on image) localises specifically to the cytoplasm of germ cells (identified by Oct4-GFP green, nuclear)
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提交于 Mar 30 2012