Anti-DDX3抗体(ab95979)
Key features and details
- Rabbit polyclonal to DDX3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-DDX3抗体
参阅全部 DDX3 一抗 -
描述
兔多克隆抗体to DDX3 -
宿主
Rabbit -
特异性
The immunogen sequence is derived from DDX3Y, the DDX3X homologous sequence is 95% identical so should also be detected byt his antibody.
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经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow -
免疫原
Recombinant fragment, corresponding to a sequence within amino acids 70-316 of Human DDX3Y.
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阳性对照
- 293T, A431, H1299, HeLa, HepG2, Molt4, and Raji cells and whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab95979于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 73 kDa.
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ICC/IF |
1/100 - 1/200.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 73 kDa. |
ICC/IF
1/100 - 1/200. |
靶标
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功能
ATP-dependent RNA helicase. Acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. Also involved in HIV-1 replication. Interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location. -
序列相似性
Belongs to the DEAD box helicase family. DDX3/DED1 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
细胞定位
Nucleus speckle. Cytoplasm. Located predominantly in nuclear speckles and, at low levels, throughout the cytoplasm. Located to the outer side of nuclear pore complexes (NPC). Shuttles between the nucleus and the cytoplasm in a XPO1-dependent manner. - Information by UniProt
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数据库链接
- Entrez Gene: 1654 Human
- Entrez Gene: 13205 Mouse
- Entrez Gene: 317335 Rat
- Omim: 300160 Human
- SwissProt: O00571 Human
- SwissProt: Q62167 Mouse
- Unigene: 380774 Human
- Unigene: 289662 Mouse
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别名
- ATP dependent RNA helicase DDX3X antibody
- ATP-dependent RNA helicase DDX3X antibody
- CAP Rf antibody
see all
图片
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All lanes : Anti-DDX3 antibody (ab95979) at 1/10000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 73 kDa -
ab95979 at 1/200 dilution staining DDX3 in HeLa cells by Immunofluorescence, Paraformaldehyde-fixed. Lower image is merged with DNA probe.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab95979 尚未被引用在任何文献中。