Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: antigen unmasking solution
Sample
Mouse Tissue sections (Testis)
Specification
Testis
Permeabilization
No
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/400
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
Detected using SuperPicture™ Kit (DAB, Rabbit)
The DAB detects Rabbit IgG using polymer technology (HRP conjugated)
PubMed ID
21641340: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Dan Stummer
Verified customer
提交于 Apr 09 2014