Application
Western blot
Sample
Mouse Cell lysate - whole cell (Neural stem cell line)
Loading amount
30 µg
Specification
Neural stem cell line
Gel Running Conditions
Reduced Denaturing (7.5%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Diluent: 5% milk TBST
Secondary antibody
Name
Non-Abcam antibody was used: Peroxidase labeled anti-goat IgG (H+L) from Vector
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 120 kDa Non-specific: 145, 90, 60 kDa
Additional data
Abcam response
Many thanks for your WB data with ab81984; in addition to detecting the specific 120kDa band, your data shows that this antibody also detects non-specific bands at 145, 90,60 kDa.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Apr 06 2010