this product to work in the species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Antigen retrieval step
Mouse Tissue sections (Embryonic mouse head, salivary glands, E13.5)
Embryonic mouse head, salivary glands, E13.5
Other product details
24 hour(s) and 0 minute(s) · Temperature: 4°C
Non-Abcam antibody was used: Alexa Fluor 594 Donkey Anti-Rabbit IgG (H+L) Anti
Host species: Rabbit
Conjugation: Alexa Fluor® 594
The concentration might need to be worked on since we got a relevant background on our samples, but we could visualize the positivity and even before trying a new concentration we believe it's true.
Abcam user community
提交于 Sep 27 2013