Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cytokeratin 7 antibody

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Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Antigen retrieval step
Heat mediated
Sample
Mouse Tissue sections (Embryonic mouse head, salivary glands, E13.5)
Specification
Embryonic mouse head, salivary glands, E13.5
Permeabilization
No
Fixative
Formaldehyde

Other product details

Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/100

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor­ 594 Donkey Anti-Rabbit IgG (H+L) Anti
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/250

Additional data

Additional Notes
The concentration might need to be worked on since we got a relevant background on our samples, but we could visualize the positivity and even before trying a new concentration we believe it's true.
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提交于 Sep 27 2013

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