Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cytokeratin 7 antibody

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Abreviews
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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Antigen retrieval step Heat mediated
Sample Mouse Tissue sections (Embryonic mouse head, salivary glands, E13.5)
Specification Embryonic mouse head, salivary glands, E13.5
Permeabilization No
Fixative Formaldehyde

Other product details

Incubation time 24 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution 1/100

Secondary antibody

Name Non-Abcam antibody was used: Alexa Fluor­ 594 Donkey Anti-Rabbit IgG (H+L) Anti
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution 1/250

Additional data

Additional Notes The concentration might need to be worked on since we got a relevant background on our samples, but we could visualize the positivity and even before trying a new concentration we believe it's true.
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提交于 Sep 27 2013