Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cytokeratin 7 antibody

Average
Abreviews
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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Bile ducts)
Specification
Bile ducts
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6
Blocking step
swine serum in TBS as blocking agent for 10 minute(s) · Concentration: 20% · Temperature: RT°C

Other product details

Dilution
1/50
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 20% swine serum in TBS

Secondary antibody

Name
Non-Abcam antibody was used: swine anti rabbit immunoglobulin
Host species: Pig
Clonality: Polyclonal
Conjugation: Other
Dilution
1/100

Additional data

Additional Notes
Antibody produces very clean results, but staining of bile ducts is weak
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提交于 Sep 23 2011

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