重组PE Anti-Cytokeratin 7抗体[EPR1619Y] - Cytoskeleton Marker (ab210628)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR1619Y] to Cytokeratin 7 - Cytoskeleton Marker
- Suitable for: ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
概述
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产品名称
PE Anti-Cytokeratin 7抗体[EPR1619Y] - Cytoskeleton Marker
参阅全部 Cytokeratin 7 一抗 -
描述
PE兔单克隆抗体[EPR1619Y] to Cytokeratin 7 - Cytoskeleton Marker -
宿主
Rabbit -
偶联物
PE. Ex: 488nm, Em: 575nm -
经测试应用
适用于: ICC/IF, Flow Cyt (Intra)more details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Flow Cyt (intra)ometry: T47D cells ICC/IF: T47D cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
存储溶液
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR1619Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-Cytokeratin 7 antibody [EPR1619Y] - BSA and Azide free (ab181831)
- Alexa Fluor® 488 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab185048)
- Alexa Fluor® 647 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab192077)
- HRP Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab192079)
- Alexa Fluor® 594 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab203427)
- Alexa Fluor® 555 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab203434)
- Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459)
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab210628于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/100.
This product gave a positive signal in T47D cells fixed with 100% methanol (5 min), and 4% formaldehyde (10 min) |
|
Flow Cyt (Intra) |
1/500.
|
说明 |
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ICC/IF
1/100. This product gave a positive signal in T47D cells fixed with 100% methanol (5 min), and 4% formaldehyde (10 min) |
Flow Cyt (Intra)
1/500. |
靶标
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功能
Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). -
组织特异性
Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus. -
序列相似性
Belongs to the intermediate filament family. -
翻译后修饰
Arg-20 is dimethylated, probably to asymmetric dimethylarginine. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 3855 Human
- Omim: 148059 Human
- SwissProt: P08729 Human
- Unigene: 411501 Human
- Unigene: 670221 Human
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别名
- CK 7 antibody
- CK-7 antibody
- CK7 antibody
see all
图片
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Figure Legend for the image: ab210628 staining Cytokeratin 7 in T74D cells. The cells were fixed with 100%methanol, permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab210628 at 1/100 dilution (pseudocolored in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in T74D cells fixed with 4%formaldehyde.
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Overlay histogram showing T47D cells stained with ab210628 (red line). The cells were fixed with 4% formaldehyde and then permeabilized with 90% methanol at -20°C for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab210628, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
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