Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (Epithelial)
Specification
Epithelial
Fixative
Acetone
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Other product details
Dilution
1/200
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Dilution
1/500
Additional data
Additional Notes
Keratin VI (Far Red), Collage IV (FITC), Nuclei (DAPI). The antibody stained the basal epithelial cells well, but there was significant cross reactivity. The antibody stained both the granulation tissue and endothelial cells.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Daniel Holod-Betz
Verified customer
提交于 May 25 2012