Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Human Cell lysate - whole cell (Human Breast Cancer Cell Lines)
Specification
Human Breast Cancer Cell Lines
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% skim milk in TBS-T
Dilution
1/10000
Secondary antibody
Name
Non-Abcam antibody was used: HRP-conjugate anti Rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Bands
Specific: 62 kDa
Exposure
1 minute(s) and 30 second(s)
Additional data
Additional Notes
Membrane had been probed with other rabbit antibodies prior to being stripped and re-probe with the Rb anti CK5. Unable to determine if there are any non-specific bands
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Mar 04 2015