Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (esophagus)
Specification
esophagus
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Blocking step
DAKO serum free protein blocker as blocking agent for 20 minute(s) · Concentration: 0.25% · Temperature: 28°C
Other product details
Dilution
1/100
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: DAKO antibody dilutent with back reducer
Secondary antibody
Name
Non-Abcam antibody was used: DAKO LSAB+
Host species: Staphylococcus aureus
Clonality: Polyclonal
Conjugation: Biotin
Host species: Staphylococcus aureus
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
Cytokeratin-20 is commonly used as a marker for intestinal metaplasia. This photo shows that some of esophageal squamous epithelial cells express Cytokeratin-20, a sign of intestinal columnar transformation, known as Barrett's esophagus. The antibody is very specific, no any background.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. J Chai
Verified customer
提交于 Apr 11 2012