Anti-Cytokeratin 19抗体(ab15463)
Key features and details
- Rabbit polyclonal to Cytokeratin 19
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Cytokeratin 19抗体
参阅全部 Cytokeratin 19 一抗 -
描述
兔多克隆抗体to Cytokeratin 19 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Synthetic peptide within Human Cytokeratin 19 aa 300 to the C-terminus. The exact sequence is proprietary.
Database link: P08727 -
常规说明
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab15463于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 40 kDa).
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IHC-P | (9) |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Boil in citrate buffer for 10 minutes, cool at room temperature for 20 minutes. After blocking, incubate primary antibody 1:100 for 10 minutes at room temperature. |
说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 40 kDa). |
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Boil in citrate buffer for 10 minutes, cool at room temperature for 20 minutes. After blocking, incubate primary antibody 1:100 for 10 minutes at room temperature. |
靶标
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功能
Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. -
组织特异性
Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin. -
序列相似性
Belongs to the intermediate filament family. -
发展阶段
Present in hair follicles at all stages of development. -
结构域
This keratin differs from all other IF proteins in lacking the C-terminal tail domain. - Information by UniProt
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数据库链接
- Entrez Gene: 3880 Human
- Entrez Gene: 16669 Mouse
- Omim: 148020 Human
- SwissProt: P08727 Human
- SwissProt: P19001 Mouse
- Unigene: 654568 Human
- Unigene: 439699 Mouse
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别名
- 40 kDa keratin intermediate filament antibody
- CK 19 antibody
- CK-19 antibody
see all
图片
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Human skin stained with anti-Cytokeratin 19 antibody (ab15463).
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All lanes : Anti-Cytokeratin 19 antibody (ab15463) at 1 µg/ml
Lane 1 : Human placenta tissue lysate - total protein (ab29745)
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 40 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands. -
ab15463 staining Cytokeratin 19 in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500 in blocking buffer) for 16 hours at 22°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse developing skin tissue labeling Cytokeratin 19 with ab15463 at 1/500 dilution. The tissue was fixed with formaldehyde; heat-mediated antigen retrieval was performed using a citric acid. Tissue was blocked with 2% BSA for 10 minutes at 21°C. A polyclonal goat anti-rabbit IgG biotin conjugated antibody was used ar 1/300 dilution. Image shows strong immunopositivity in the cytoplasm of basal cells of the epidermis and a moderate hair follicle cell positivity.
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This image shows formalin-fixed mouse skin stained with Cytokeratin 19 antibody (1/50 dilution) after pepsin digestion.
数据表及文件
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SDS download
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Datasheet download
文献 (74)
ab15463 被引用在 74 文献中.
- Polvani S et al. Isoforms of the orphan nuclear receptor COUP‑TFII differentially modulate pancreatic cancer progression. Int J Oncol 60:N/A (2022). PubMed: 35348189
- Sun Y et al. Diet-Induced Obesity Promotes Liver Metastasis of Pancreatic Ductal Adenocarcinoma via CX3CL1/CX3CR1 Axis. J Immunol Res 2022:5665964 (2022). PubMed: 35478937
- Di Lorenzo A et al. Toll-like receptor 2 promotes breast cancer progression and resistance to chemotherapy. Oncoimmunology 11:2086752 (2022). PubMed: 35756841
- Glaubitz J et al. In mouse chronic pancreatitis CD25+FOXP3+ regulatory T cells control pancreatic fibrosis by suppression of the type 2 immune response. Nat Commun 13:4502 (2022). PubMed: 35922425
- Monzer A et al. Novel therapeutic diiminoquinone exhibits anticancer effects on human colorectal cancer cells in two-dimensional and three-dimensional in vitro models. World J Gastroenterol 28:4787-4811 (2022). PubMed: 36156922