重组PerCP荧光Anti-Cytokeratin 19抗体[EPR1579Y] (ab221256)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PerCP Rabbit monoclonal [EPR1579Y] to Cytokeratin 19
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: PerCP. Ex: 482nm, Em: 675nm
Related conjugates and formulations
概述
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产品名称
PerCP荧光Anti-Cytokeratin 19抗体[EPR1579Y]
参阅全部 Cytokeratin 19 一抗 -
描述
PerCP荧光兔单克隆抗体[EPR1579Y] to Cytokeratin 19 -
宿主
Rabbit -
偶联物
PerCP. Ex: 482nm, Em: 675nm -
经测试应用
适用于: Flow Cyt (Intra)more details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human Cytokeratin 19 (N terminal). The exact sequence is proprietary.
Database link: P08727 -
阳性对照
- Flow Cyt (intra): MCF7 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR1579Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EPR1579Y] (ab205445)
- Alexa Fluor® 647 Anti-Cytokeratin 19 antibody [EPR1579Y] (ab205446)
- PE Anti-Cytokeratin 19 antibody [EPR1579Y] (ab216705)
- APC Anti-Cytokeratin 19 antibody [EPR1579Y] (ab221255)
- Anti-Cytokeratin 19 antibody [EPR1579Y] - BSA and Azide free (ab232566)
- Anti-Cytokeratin 19 antibody [EPR1579Y] (ab76539)
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab221256于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/50.
The cellular localisation of this product has been verified in ICC/IF.ab222107 - Rabbit monoclonal IgG (PerCP), is suitable for use as an isotype control with this antibody. |
说明 |
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Flow Cyt (Intra)
1/50. The cellular localisation of this product has been verified in ICC/IF.ab222107 - Rabbit monoclonal IgG (PerCP), is suitable for use as an isotype control with this antibody. |
靶标
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功能
Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. -
组织特异性
Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin. -
序列相似性
Belongs to the intermediate filament family. -
发展阶段
Present in hair follicles at all stages of development. -
结构域
This keratin differs from all other IF proteins in lacking the C-terminal tail domain. - Information by UniProt
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数据库链接
- Entrez Gene: 3880 Human
- Omim: 148020 Human
- SwissProt: P08727 Human
- Unigene: 654568 Human
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别名
- 40 kDa keratin intermediate filament antibody
- CK 19 antibody
- CK-19 antibody
see all
图片
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Overlay histogram showing MCF7 cells stained with ab221256 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab221256, 1/50 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) PerCP (ab222107) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 685/35 bandpass filter.
This antibody gave a positive signal in MCF7 cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
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