FITC荧光Anti-Cytokeratin 18抗体[DC-10] (ab72813)
Key features and details
- FITC Mouse monoclonal [DC-10] to Cytokeratin 18
- Suitable for: ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
概述
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产品名称
FITC荧光Anti-Cytokeratin 18抗体[DC-10]
参阅全部 Cytokeratin 18 一抗 -
描述
FITC荧光小鼠单克隆抗体[DC-10] to Cytokeratin 18 -
宿主
Mouse -
偶联物
FITC. Ex: 493nm, Em: 528nm -
经测试应用
适用于: ICC/IF, Flow Cyt (Intra)more details -
种属反应性
与反应: Human
不与反应: Mouse, Rat, Sheep, Hamster, Cow, Dog, Pig -
免疫原
Tissue, cells or virus corresponding to Human Cytokeratin 18. Human breast carcinoma cell line PMC-42
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阳性对照
- ICC/IF: HCT116 cells. Flow Cyt (Intra): HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.4
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Size exclusion -
纯化说明
The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC. -
克隆
单克隆 -
克隆编号
DC-10 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72813于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 1 µg/ml.
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Flow Cyt (Intra) |
Use a concentration of 1 - 5 µg/ml.
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说明 |
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ICC/IF
Use a concentration of 1 µg/ml. |
Flow Cyt (Intra)
Use a concentration of 1 - 5 µg/ml. |
靶标
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功能
Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. -
组织特异性
Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma. -
疾病相关
Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600]. -
序列相似性
Belongs to the intermediate filament family. -
翻译后修饰
Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues. -
细胞定位
Cytoplasm > perinuclear region. - Information by UniProt
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数据库链接
- Entrez Gene: 3875 Human
- Omim: 148070 Human
- SwissProt: P05783 Human
- Unigene: 406013 Human
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别名
- Cell proliferation inducing gene 46 protein antibody
- Cell proliferation inducing protein 46 antibody
- Cell proliferation-inducing gene 46 protein antibody
see all
图片
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Flow cytometry analysis (intracellular staining) of HeLa cells with ab72813 at 5 µg/ml.
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Overlay histogram showing MCF-7 cells stained with ab72813 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab72813, 0.5µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 (FITC) (2µg/1x106 cells) for 30 min at 22°C. Acquisition of >5,000 events was performed.
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ICC/IF image of ab72813 stained HCT116 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72813, 1µg/ml) overnight at +4°C. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab72813 被引用在 3 文献中.
- Zhang Q et al. Isolation of circulating tumor cells and detection of EGFR mutations in patients with non-small-cell lung cancer. Oncol Lett 17:3799-3807 (2019). PubMed: 30881500
- Zhang H et al. A liquid biopsy-based method for the detection and quantification of circulating tumor cells in surgical osteosarcoma patients. Int J Oncol N/A:N/A (2017). PubMed: 28350107
- Wagner BJ et al. Phagocytosis of dying tumor cells by human peritoneal mesothelial cells. J Cell Sci 124:1644-54 (2011). PubMed: 21525033