Anti-Cytokeratin 18抗体[DA-7] (Biotin) (ab27549)

概述

  • 产品名称
    Anti-Cytokeratin 18抗体[DA-7] (Biotin)
    参阅全部 Cytokeratin 18 一抗
  • 描述
    小鼠单克隆抗体[DA-7] to Cytokeratin 18 (Biotin)
  • 偶联物
    Biotin
  • 特异性
    ab27549 recognises Cytokeratin peptide 18. We have data to indicate that this antibody may not cross react with wide range of other species. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • 经测试应用
    适用于: WB, ELISA, ICC, IHC-P, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Human breast carcinoma cell line PMC-42.

  • 阳性对照
    • Western blot and immunocytochemistry: HeLa human cervix carcinoma cell line, MCF-7 human breast adenocarcinoma cell line. Immunohistochemistry: epithelial tissue (skin). ELISA: MCF-7 human breast adenocarcinoma cell line

性能

应用

Our Abpromise guarantee covers the use of ab27549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Predicted molecular weight: 45 kDa.
ELISA Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution.
IP Use at an assay dependent dilution.

靶标

  • 功能
    Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
  • 组织特异性
    Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
  • 疾病相关
    Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
  • 序列相似性
    Belongs to the intermediate filament family.
  • 翻译后修饰
    Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
  • 细胞定位
    Cytoplasm > perinuclear region.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cell proliferation inducing gene 46 protein antibody
    • Cell proliferation inducing protein 46 antibody
    • Cell proliferation-inducing gene 46 protein antibody
    • CK 18 antibody
    • CK-18 antibody
    • CK18 antibody
    • CYK 18 antibody
    • CYK18 antibody
    • Cytokeratin 18 antibody
    • Cytokeratin endo B antibody
    • Cytokeratin-18 antibody
    • K 18 antibody
    • K18 antibody
    • K1C18_HUMAN antibody
    • KA18 antibody
    • Keratin 18 antibody
    • Keratin 18, type I antibody
    • Keratin D antibody
    • keratin, type I cytoskeletal 18 antibody
    • Keratin-18 antibody
    • Krt18 antibody
    see all

图片

  • Immunocytochemistry/Immunofluorescence analysis of human ovarian tumour tissue labelling Cytokeratin 18 with ab7549 conjugated to conjugated to QD655 at a molar ratio of 6:1 (Ab:QD). Tissue was incubated with primary antibody overnight at 4°C. Right image - tissue was incubated a further 2 hours at room temperature in order to remove background staining.

  • Anti-Cytokeratin 18 antibody [DA-7] (Biotin) (ab27549) at 1 µg/ml + HeLa (50 million) cells re-suspended in 1 ml cold Lysis buffer.

    Performed under non-reducing conditions.

    Predicted band size : 45 kDa

文献

This product has been referenced in:
  • Sweeney E  et al. Quantitative multiplexed quantum dot immunohistochemistry. Biochem Biophys Res Commun 374:181-6 (2008). ICC ; Human . Read more (PubMed: 18621021) »

See 1 Publication for this product

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