Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Pig Tissue sections (Small intestine)
Specification
Small intestine
Permeabilization
No
Fixative
Formaldehyde
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/700
Secondary antibody
Name
Non-Abcam antibody was used: anti Mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
Submitted image shows staining restricted to Goblet cell cytoplasm. Enterocyte cytoplasm should be positive.
Abcam response
Thank you very much for your valuable feedback and your time to test this antibody in IHC on Pig tissue sections. Your data suggests that under these experimental conditions ab668 may not be suitable to detect Cytokeratin 18 in Pig small intestine tissue sections. We welcome further customer feedback. We hope this information is useful to our customers. For more information, please contact scientific support.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
提交于 Sep 11 2014