重组Anti-Cytokeratin 16/K16抗体[EP1615Y] (ab76416)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1615Y] to Cytokeratin 16/K16
- Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Cytokeratin 16/K16抗体[EP1615Y]
参阅全部 Cytokeratin 16/K16 一抗 -
描述
兔单克隆抗体[EP1615Y] to Cytokeratin 16/K16 -
宿主
Rabbit -
特异性
Several customers have found that this antibody gives good results in mouse and rat however in our hands, we cannot obtain positive results. This antibody is therefore no longer covered by our Abpromise guarantee for use in mouse or rat.
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经测试应用
适用于: Flow Cyt (Intra), IHC-P, WB, ICC/IFmore details
不适用于: IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human Cytokeratin 16/K16 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P08779 -
阳性对照
- HaCat cell lysate; human squamous cervical carcinoma tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1615Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Alexa Fluor® 488 Anti-Cytokeratin 16/K16 antibody [EP1615Y] (ab205744)
- Alexa Fluor® 647 Anti-Cytokeratin 16/K16 antibody [EP1615Y] (ab205745)
- HRP Anti-Cytokeratin 16/K16 antibody [EP1615Y] (ab206064)
- Alexa Fluor® 555 Anti-Cytokeratin 16/K16 antibody [EP1615Y] (ab215292)
- Anti-Cytokeratin 16/K16 antibody [EP1615Y] - BSA and Azide free (ab232522)
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76416于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
1/50 - 1/150.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IHC-P | (2) |
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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WB |
1/5000 - 1/10000. Predicted molecular weight: 51 kDa.
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ICC/IF |
1/100 - 1/150.
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说明 |
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Flow Cyt (Intra)
1/50 - 1/150. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
WB
1/5000 - 1/10000. Predicted molecular weight: 51 kDa. |
ICC/IF
1/100 - 1/150. |
靶标
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组织特异性
Expressed in the hair follicle, nail bed and in mucosal stratified squamous epithelia and, suprabasally, in oral epithelium and palmoplantar epidermis. Also found in luminal cells of sweat and mammary gland ducts. -
疾病相关
Defects in KRT16 are a cause of pachyonychia congenita type 1 (PC1) [MIM:167200]; also known as Jadassohn-Lewandowsky syndrome. PC1 is an autosomal dominant ectodermal dysplasia characterized by hypertrophic nail dystrophy resulting in onchyogryposis (thickening and increase in curvature of the nail), palmoplantar keratoderma, follicular hyperkeratosis, and oral leukokeratosis. Hyperhidrosis of the hands and feet is usually present.
Defects in KRT16 are the cause of palmoplantar keratoderma non-epidermolytic focal (FNEPPK) [MIM:613000]. A dermatological disorder characterized by non-epidermolytic palmoplantar keratoderma limited to the pressure points on the balls of the feet, with later mild involvement on the palms. Oral, genital and follicular keratotic lesions are often present.
Defects in KRT16 are a cause of unilateral palmoplantar verrucous nevus (UPVN) [MIM:144200]. UPVN is characterized by a localized thickening of the skin in parts of the right palm and the right sole.
Note=KRT16 and KRT17 are coexpressed only in pathological situations such as metaplasias and carcinomas of the uterine cervix and in psoriasis vulgaris. -
序列相似性
Belongs to the intermediate filament family. - Information by UniProt
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数据库链接
- Entrez Gene: 3868 Human
- Omim: 148067 Human
- SwissProt: P08779 Human
- Unigene: 655160 Human
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别名
- CK 16 antibody
- CK-16 antibody
- CK16 antibody
see all
图片
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ab76416 staining Cytokeratin 16/K16 in human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.
Negative control 1: PBS in place of primary antibody.
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Anti-Cytokeratin 16/K16 antibody [EP1615Y] (ab76416) at 1/10000 dilution + HACAT (human keratinocyte) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051 at 1/20000 dilution
Predicted band size: 51 kDa -
ab76416 staining Cytokeratin 16/K16 in A431 (human epidermoid carcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody. ab7291 and ab150120 were used as counterstains for primary antibody ab75748 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.
Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077) -
Overlay histogram showing HepG2 cells stained with unpurified ab76416 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS/10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76416, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Triton used under the same conditions.
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ab76416 staining Cytokeratin 16/K16 in HACAT (human keratinocyte) cell lineby intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/150. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/500 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
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ab76416 staining Cytokeratin 16/K16 in human skeletal muscle sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.
Negative control 1: PBS in place of primary antibody.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (19)
ab76416 被引用在 19 文献中.
- Hou X et al. 3D-SeboSkin Model for Human ex vivo Studies of Hidradenitis Suppurativa/Acne Inversa. Dermatology 238:236-243 (2022). PubMed: 34077928
- Li Z et al. ESR1 mutant breast cancers show elevated basal cytokeratins and immune activation. Nat Commun 13:2011 (2022). PubMed: 35440136
- Jung KL et al. Single-cell analysis of Kaposi's sarcoma-associated herpesvirus infection in three-dimensional air-liquid interface culture model. PLoS Pathog 18:e1010775 (2022). PubMed: 35976902
- Laly AC et al. The keratin network of intermediate filaments regulates keratinocyte rigidity sensing and nuclear mechanotransduction. Sci Adv 7:N/A (2021). PubMed: 33571121
- Lossius AH et al. Early transcriptional changes after UVB treatment in atopic dermatitis include inverse regulation of IL-36γ and IL-37. Exp Dermatol 30:249-261 (2021). PubMed: 33067891