FITC荧光Anti-Cytokeratin 14抗体[LL002] (ab77684)
Key features and details
- FITC Mouse monoclonal [LL002] to Cytokeratin 14
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG3
Related conjugates and formulations
概述
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产品名称
FITC荧光Anti-Cytokeratin 14抗体[LL002]
参阅全部 Cytokeratin 14 一抗 -
描述
FITC荧光小鼠单克隆抗体[LL002] to Cytokeratin 14 -
宿主
Mouse -
偶联物
FITC. Ex: 493nm, Em: 528nm -
经测试应用
适用于: IHC-P, Flow Cytmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide: GKVVSTHEQVLRTKN conjugated to Thyroglobulin, corresponding to C terminal amino acids 458-472 of Human Cytokeratin 14
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Store at +4°C. -
存储溶液
Preservative: 0.065% Sodium azide
Constituents: 0.1% BSA, PBS -
Concentration information loading...
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纯度
Ion Exchange Chromatography -
纯化说明
Ammonium sulphate precipitation followed by ion exchange chromatography. -
克隆
单克隆 -
克隆编号
LL002 -
骨髓瘤
NS1 -
同种型
IgG3 -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab77684于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P | (1) |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
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Flow Cyt |
Use 10µl for 106 cells.
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说明 |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. |
Flow Cyt
Use 10µl for 106 cells. |
靶标
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功能
The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro. -
组织特异性
Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen. -
疾病相关
Defects in KRT14 are a cause of epidermolysis bullosa simplex Dowling-Meara type (DM-EBS) [MIM:131760]. DM-EBS is a severe form of intraepidermal epidermolysis bullosa characterized by generalized herpetiform blistering, milia formation, dystrophic nails, and mucous membrane involvement.
Defects in KRT14 are a cause of epidermolysis bullosa simplex Weber-Cockayne type (WC-EBS) [MIM:131800]. WC-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering limited to palmar and plantar areas of the skin.
Defects in KRT14 are a cause of epidermolysis bullosa simplex Koebner type (K-EBS) [MIM:131900]. K-EBS is a form of intraepidermal epidermolysis bullosa characterized by generalized skin blistering. The phenotype is not fundamentally distinct from the Dowling-Meara type, although it is less severe.
Defects in KRT14 are the cause of epidermolysis bullosa simplex autosomal recessive (AREBS) [MIM:601001]. AREBS is an intraepidermal epidermolysis bullosa characterized by localized blistering on the dorsal, lateral and plantar surfaces of the feet.
Defects in KRT14 are the cause of Naegeli-Franceschetti-Jadassohn syndrome (NFJS) [MIM:161000]; also known as Naegeli syndrome. NFJS is a rare autosomal dominant form of ectodermal dysplasia. The cardinal features are absence of dermatoglyphics (fingerprints), reticular cutaneous hyperpigmentation (starting at about the age of 2 years without a preceding inflammatory stage), palmoplantar keratoderma, hypohidrosis with diminished sweat gland function and discomfort provoked by heat, nail dystrophy, and tooth enamel defects.
Defects in KRT14 are the cause of dermatopathia pigmentosa reticularis (DPR) [MIM:125595]. DPR is a rare ectodermal dysplasia characterized by lifelong persistent reticulate hyperpigmentation, noncicatricial alopecia, and nail dystrophy. -
序列相似性
Belongs to the intermediate filament family. -
细胞定位
Cytoplasm. Nucleus. Expressed in both as a filamentous pattern. - Information by UniProt
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数据库链接
- Entrez Gene: 3861 Human
- Entrez Gene: 16664 Mouse
- Entrez Gene: 287701 Rat
- Omim: 148066 Human
- SwissProt: P02533 Human
- SwissProt: Q61781 Mouse
- SwissProt: Q6IFV1 Rat
- Unigene: 654380 Human
see all -
别名
- CK 14 antibody
- CK-14 antibody
- ck14 antibody
see all
图片
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Overlay histogram showing A431 cells stained with ab77684 (red line). The cells were fixed with 4% paraformaldehyde (10 min)) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77684, 1µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG3 FITC (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 80% methanol/permeabilized in 0.1% PBS-Triton used under the same conditions.
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IHC image of ab77684 staining in human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77684, neat, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (12)
ab77684 被引用在 12 文献中.
- Royzman D et al. Soluble CD83 improves and accelerates wound healing by the induction of pro-resolving macrophages. Front Immunol 13:1012647 (2022). PubMed: 36248909
- Wei F et al. Plasma endothelial cells-derived extracellular vesicles promote wound healing in diabetes through YAP and the PI3K/Akt/mTOR pathway. Aging (Albany NY) 12:12002-12018 (2020). PubMed: 32570219
- Szymanski L et al. A Simple Method for the Production of Human Skin Equivalent in 3D, Multi-Cell Culture. Int J Mol Sci 21:N/A (2020). PubMed: 32629914
- Crowell PD et al. Expansion of Luminal Progenitor Cells in the Aging Mouse and Human Prostate. Cell Rep 28:1499-1510.e6 (2019). PubMed: 31390564
- Halim NS et al. The effect of mesenchymal stem cell-secreted factors on airway epithelial repair. Regen Med N/A:N/A (2018). PubMed: 30566028