Anti-Cytokeratin 1抗体[AE1] (ab9286)
Key features and details
- Mouse monoclonal [AE1] to Cytokeratin 1
- Suitable for: IHC-P, IHC-Fr, WB
- Reacts with: Mouse, Rat, Rabbit, Chicken, Cow, Monkey, Turtle
- Isotype: IgG1
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Cytokeratin 1抗体[AE1]
参阅全部 Cytokeratin 1 一抗 -
描述
小鼠单克隆抗体[AE1] to Cytokeratin 1 -
宿主
Mouse -
特异性
This antibody recognises most of the acidic (Type 1) keratins. -
经测试应用
适用于: IHC-P, IHC-Fr, WBmore details -
种属反应性
与反应: Mouse, Rat, Rabbit, Chicken, Cow, Monkey, Turtle -
免疫原
Full length native protein (purified) corresponding to Human Cytokeratin 1. Epidermal keratin
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.3
Preservative: 0.1% Sodium azide -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
AE1 -
骨髓瘤
unknown -
同种型
IgG1 -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
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IHC-P | (2) |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 66 kDa.
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说明 |
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IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 66 kDa. |
靶标
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功能
May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein kinase C (RACK1/GNB2L1). -
组织特异性
The source of this protein is neonatal foreskin. The 67-kDa type II keratins are expressed in terminally differentiating epidermis. -
疾病相关
Defects in KRT1 are a cause of bullous congenital ichthyosiform erythroderma (BCIE) [MIM:113800]; also known as epidermolytic hyperkeratosis (EHK) or bullous erythroderma ichthyosiformis congenita of Brocq. BCIE is an autosomal dominant skin disorder characterized by widespread blistering and an ichthyotic erythroderma at birth that persist into adulthood. Histologically there is a diffuse epidermolytic degeneration in the lower spinous layer of the epidermis. Within a few weeks from birth, erythroderma and blister formation diminish and hyperkeratoses develop.
Defects in KRT1 are the cause of ichthyosis hystrix Curth-Macklin type (IHCM) [MIM:146590]. IHCM is a genodermatosis with severe verrucous hyperkeratosis. Affected individuals manifest congenital verrucous black scale on the scalp, neck, and limbs with truncal erythema, palmoplantar keratoderma and keratoses on the lips, ears, nipples and buttocks.
Defects in KRT1 are a cause of palmoplantar keratoderma non-epidermolytic (NEPPK) [MIM:600962]. NEPKK is a dermatological disorder characterized by focal palmoplantar keratoderma with oral, genital, and follicular lesions.
Defects in KRT1 are a cause of ichthyosis annular epidermolytic (AEI) [MIM:607602]; also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI is a skin disorder resembling bullous congenital ichthyosiform erythroderma. Affected individuals present with bullous ichthyosis in early childhood and hyperkeratotic lichenified plaques in the flexural areas and extensor surfaces at later ages. The feature that distinguishes AEI from BCIE is dramatic episodes of flares of annular polycyclic plaques with scale, which coalesce to involve most of the body surface and can persist for several weeks or even months.
Defects in KRT1 are the cause of palmoplantar keratoderma striate type 3 (SPPK3) [MIM:607654]; also known as keratosis palmoplantaris striata III. SPPK3 is a dermatological disorder affecting palm and sole skin where stratum corneum and epidermal layers are thickened. There is no involvement of non-palmoplantar skin, and both hair and nails are normal. -
序列相似性
Belongs to the intermediate filament family. -
翻译后修饰
Undergoes deimination of some arginine residues (citrullination). -
细胞定位
Cell membrane. Located on plasma membrane of neuroblastoma NMB7 cells. - Information by UniProt
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数据库链接
- Entrez Gene: 100301161 Cow
- Entrez Gene: 281266 Cow
- Entrez Gene: 16678 Mouse
- Entrez Gene: 300250 Rat
- SwissProt: P04104 Mouse
- SwissProt: Q6IMF3 Rat
- Unigene: 183137 Mouse
- Unigene: 31789 Rat
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别名
- 67 kDa cytokeratin antibody
- CK-1 antibody
- CK1 antibody
see all
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab9286 被引用在 13 文献中.
- Choo MK et al. The protein kinase p38a destabilizes p63 to limit epidermal stem cell frequency and tumorigenic potential. Sci Signal 11:N/A (2018). PubMed: 30301786
- Fang H et al. Proinflammatory role of blister fluid-derived exosomes in bullous pemphigoid. J Pathol 245:114-125 (2018). PubMed: 29468680
- Yin C et al. TLR7-expressing cells comprise an interfollicular epidermal stem cell population in murine epidermis. Sci Rep 4:5831 (2014). PubMed: 25060222
- Caballero-Franco C et al. Tuning of protein kinase circuitry by p38a is vital for epithelial tissue homeostasis. J Biol Chem 288:23788-97 (2013). PubMed: 23836897
- Spagnolo DV et al. Monoclonal anti-keratin (AE1) reactivity in routinely processed tissue from 166 human neoplasms. Am J Clin Pathol 84:697-704 (1985). PubMed: 2416215