Application
Western blot
Sample
Pig Tissue lysate - other (liver microsomal protein)
Loading amount
20 µg
Specification
liver microsomal protein
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/5000
Incubation time
1 hour(s) and 0 minute(s) · Diluent: 5% milk_TBST
Secondary antibody
Name
Non-Abcam antibody was used: anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
supersignal PICO
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: around 55 kDa
Additional data
Additional Notes
Only one band can be seen in western blot by this rat 3A1 antibody and equal size comparing to the human 3A4 antibody.
Abcam response
Thank you for your valuable feedback. We are delighted to hear that the antibody works on pig samples, as this species had not previously been tested with the product.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Ying Chen
Verified customer
提交于 Jun 15 2007