Application
Western blot
Sample
Mouse Tissue lysate - other (Liver microsomes)
Loading amount
10 µg
Specification
Liver microsomes
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/2500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-mouse IgG(H+L)-HRP conjugate
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL plus
Exposure
1 second(s)
Bands
Specific: 55 kDa
Positive control
lane 1-4: normal mouse liver tissue
Additional data
Additional Notes
1:2500 was still too strong, later I used 1:5000, which gave nicer band.
Lanes 1-4: normal mouse liver tissue.
Lanes 1-4: normal mouse liver tissue.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Aug 01 2011