Application
Western blot
Sample
Human Cell lysate - whole cell (Epithelial OVCA cell line)
Gel Running Conditions
Reduced Denaturing (12.5)
Loading amount
100 µg
Treatment
0- 200µM Perifosine 24 hr
Specification
Epithelial OVCA cell line
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% milk
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Anti-Rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL Western Blotting Substrate
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 15 kDa
Positive control
Untreated OVACAR cell extract
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Aug 09 2016