Application
Western blot
Sample
Mouse Tissue lysate - other (Cytosolic fractions from GTN skeletal muscles)
Loading amount
50 µg
Specification
Cytosolic fractions from GTN skeletal muscles
Gel Running Conditions
Reduced Denaturing (15% gel)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Other product details
Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% milk in TBS-Tween (0.05%)
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Mouse IgG, HRP-linked Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
30 second(s)
Bands
Specific: 15 kDa
Additional data
Additional Notes
Nitrocellulose membrane
1% SDS lysis buffer with 1:100 protease inhibitors
1% SDS lysis buffer with 1:100 protease inhibitors
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. G.K Sakellariou
Verified customer
提交于 Sep 28 2012