F-actin Staining试剂盒- Red Fluorescence - Cytopainter (ab112127)
Key features and details
- Assay type: Cell-based
- Platform: Fluorescence microscope
- Sample type: Adherent cells, Suspension cells, Tissue
概述
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产品名称
F-actin Staining试剂盒- Red Fluorescence - Cytopainter
参阅全部 F-actin 试剂盒 -
样品类型
Tissue, Adherent cells, Suspension cells -
检测类型
Cell-based -
种属反应性
与反应: Mammals, Other species -
产品概述
F-actin Staining Kit - Red Fluorescence | Cytopainter (ab112127) fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.
ab112127 is designed to stain F-actins in fixed cells with red fluorescence. The red fluorescent phalloidin conjugate, which is selectively bound to F-actins, is a high-affinity probe for F-actins. The red fluorescent phalloidin conjugate has Ex/Em = 594/610 nm. Used at nanomolar concentrations, phallotoxins can be conveniently used to label, identify and quantitate F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. The red fluorescent phalloidin conjugate has good thermal and photo stability.
ab112127 provides all the essential components with an optimized labeling protocol. It is an excellent tool for preserving fluorescent images of particular cells, and can also be used for fluorescence microscope demonstrations.
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说明
ab112127 should be stored dessicated.
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平台
Fluorescence microscope
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 标识符 500 tests Labeling Buffer 1 x 50ml Red Fluorescent Phalloidin Conjugate Component A 1 vial -
研究领域
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别名
- actin filament
- f actin
- Filamentous actin
图片
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F-actin staining (red) in chondrocytes in growth plate cartilage. Mouse femur bone was fixed with 4% PFA for 72 hours, decalcified and cut into 8-10 µm sections in frozen. Tissue was then stained for 60 minutes with ab112127 before a final wash in PBS.
This image is courtesy of an anonymous Abreview
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HeLa cells were fixed with 4% PFA for 10 minutes, rinsed with PBS and stained for 60 minutes with ab112127 before a final wash in PBS prior to mounting slides. Images obtained with an Olympus BX61 microscope using a Texas Red filter (595/613nm) - 50ms exposure.
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Images of CPA cells fixed with formaldehyde and stained with ab112127 in a black 96-well plate Left image: Cells labeled with 1X Red Fluorescent Phalloidin Conjugate for 30 min only. Right image: Cells treated with phalloidin for 10 min, then stained with Red Fluorescent Phalloidin Conjugate for 30 min.
数据表及文件
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SDS download
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Datasheet download
文献 (18)
ab112127 被引用在 18 文献中.
- Tao M et al. Decreased RNA m6A methylation enhances the process of the epithelial mesenchymal transition and vasculogenic mimicry in glioblastoma. Am J Cancer Res 12:893-906 (2022). PubMed: 35261810
- Lou X et al. VIP Stabilizes the Cytoskeleton of Schlemm's Canal Endothelia via Reducing Caspase-3 Mediated ZO-1 Endolysosomal Degradation. Oxid Med Cell Longev 2021:9397960 (2021). PubMed: 34552687
- Moussa HI et al. Limitation in Controlling the Morphology of Mammalian Vero Cells Induced by Cell Division on Asymmetric Tungsten-Silicon Oxide Nanocomposite. Materials (Basel) 13:N/A (2020). PubMed: 31940759
- Luo B et al. Vagus nerve stimulation optimized cardiomyocyte phenotype, sarcomere organization and energy metabolism in infarcted heart through FoxO3A-VEGF signaling. Cell Death Dis 11:971 (2020). PubMed: 33184264
- Yan X et al. VIP Induces Changes in the F-/G-Actin Ratio of Schlemm's Canal Endothelium via LRRK2 Transcriptional Regulation. Invest Ophthalmol Vis Sci 61:45 (2020). PubMed: 32572455