Application
Western blot
Sample
Human Cell lysate - whole cell (PERIPHERAL BLOOD MONONUCLEAR CELLS)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
60 µg
Specification
PERIPHERAL BLOOD MONONUCLEAR CELLS
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA
Dilution
1/200
Secondary antibody
Dilution
1/4000
Name
Non-Abcam antibody was used: DAKO GOAT ANTI RABBIT
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
15 minute(s) and 0 second(s)
Bands
Specific: 13 kDa kDa
Positive control
u87 cell lysate, hepG2 cell lysate
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Siddharth Mehra
Verified customer
提交于 Jun 06 2016