Anti-Cyclin T2/CCNT2抗体[2128C1a] (ab50979)
Key features and details
- Mouse monoclonal [2128C1a] to Cyclin T2/CCNT2
- Suitable for: WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
概述
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产品名称
Anti-Cyclin T2/CCNT2抗体[2128C1a]
参阅全部 Cyclin T2/CCNT2 一抗 -
描述
小鼠单克隆抗体[2128C1a] to Cyclin T2/CCNT2 -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human, Recombinant fragment -
免疫原
Recombinant fragment corresponding to Human Cyclin T2/CCNT2 (C terminal).
Database link: O60583 -
表位
ab50979 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. -
常规说明
This antibody recognizes Cyclin T2a and T2b since it is made by immunogen-peptide that is common domain from both protein.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, 1% BSA, PBS -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
2128C1a -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab50979于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 81 kDa.
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说明 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 81 kDa. |
靶标
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功能
Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin T) complex, also called positive transcription elongation factor B (P-TEFB), which is proposed to facilitate the transition from abortive to production elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNAP II). -
组织特异性
Ubiquitously expressed. -
序列相似性
Belongs to the cyclin family. Cyclin C subfamily. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 905 Human
- Omim: 603862 Human
- SwissProt: O60583 Human
- Unigene: 658324 Human
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别名
- CCNT2 antibody
- CCNT2_HUMAN antibody
- Cyclin T2a antibody
see all
图片
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Anti-Cyclin T2/CCNT2 antibody [2128C1a] (ab50979) + recombinant protein fragment used as immunogen.
Diagram underneath blot depicts region of Cyclin T2 protein that was employed as immunogen (arrow).
Predicted band size: 81 kDa -
All lanes : Anti-Cyclin T2/CCNT2 antibody [2128C1a] (ab50979)
Lane 1 : Molecular Weight Marker
Lane 2 : 50µg Hela Whole cell lysate
Lane 3 : 50µg HT-1080 Whole cell lysate
Lane 4 : 50 µg HEK293 Whole cell lysate
Lane 5 : 50µg A2058 Whole cell lysate
Lane 6 : 50µg HIH3T3 Whole cell Lysate
Secondary
Lanes 2-6 : Mouse IgG Antibody 1/2500
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 81 kDa
Exposure time: 1 minute
8% SDS-PAGE gel;
a- Isoform a = 74kDa (663 a.a.);
b - Isoform b = 81kDa (730 a.a.)
数据表及文件
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Datasheet download
文献 (3)
ab50979 被引用在 3 文献中.
- Donnio LM et al. CSB-Dependent Cyclin-Dependent Kinase 9 Degradation and RNA Polymerase II Phosphorylation during Transcription-Coupled Repair. Mol Cell Biol 39:N/A (2019). PubMed: 30602496
- Zhang H et al. SIRT2 directs the replication stress response through CDK9 deacetylation. Proc Natl Acad Sci U S A 110:13546-51 (2013). PubMed: 23898190
- Wang XS et al. MicroRNA-29a and microRNA-142-3p are regulators of myeloid differentiation and acute myeloid leukemia. Blood 119:4992-5004 (2012). WB ; Human . PubMed: 22493297