Application
Western blot
Sample
Rat Tissue lysate - whole (Liver)
Loading amount
50 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Swine anti-rabbit
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 45 kDa
Additional data
Abcam response
This antibody has been tested and found to be negative for rat samples previously, we would therefore be very interested to hear from any other researches who have also used this antibody successfully with rat specimen.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Oct 28 2011