Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Loading amount
20 µg
Specification
Mouse embryonic fibroblasts
Gel Running Conditions
Reduced Denaturing (10% Tris-Gly gel)
Blocking step
LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Other product details
Dilution
1/1000
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 50mMTris,pH8.0,50mMKCl,1mMEDTA,5%glycero
Secondary antibody
Name
Non-Abcam antibody was used: IR Dye 800 goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
Odyssey® scanner
Exposure
2 minute(s) and 0 second(s)
Positive control
WCE
Negative control
-
Additional data
Additional Notes
Picture legend
Mouse embryonic fibroblasts Whole Cell Extract (20 & 40 µg)
No detectable bands were observed.
Mouse embryonic fibroblasts Whole Cell Extract (20 & 40 µg)
No detectable bands were observed.
Abcam response
The antibody ab33911 had not previously been tested on mouse tissue and so we did not know whether it would work or not. The data provided by the customer is valuable information on the activity of the antibody
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Nov 10 2011