Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Sample
Human Cell (Osteosarcoma)
Specification
Osteosarcoma
Permeabilization
Yes - triton X-100
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Goat serum (3%)
Dilution
1/50
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-mouse IgG-Alexafluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500
Additional data
Additional Notes
no signal
Abcam response
Thank you for youre review of this untested application. We value your feedback and appreicate the time yoou have taken to test the antibody and submit the review.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jul 15 2014