Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Specification
U2OS
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2 % Triton X 100 in PBS for 12 min
Blocking step
Goat serum in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Other product details
Dilution
1/500
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 10% goat serum in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 488 goat anti-mouse IgG from Invitroge
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500
Additional data
Additional Notes
Telomeres were visualized by FISH using a telomeric PNA probe labelled with Cy3. Cy3 was detected using the anti-Cy3/Cy5 antibody.
Image: red: Telomere-Cy3, green: anti-Cy3/Cy5-Alexa488.
Image: red: Telomere-Cy3, green: anti-Cy3/Cy5-Alexa488.
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提交于 Feb 25 2013