重组Anti-Cullin 4A/CUL-4A抗体[EPR3198] (ab92554)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3198] to Cullin 4A/CUL-4A
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Cullin 4A/CUL-4A抗体[EPR3198]
参阅全部 Cullin 4A/CUL-4A 一抗 -
描述
兔单克隆抗体[EPR3198] to Cullin 4A/CUL-4A -
宿主
Rabbit -
特异性
The immunogen used for this product shares 93% homology with Cul4B. Cross-reactivity with Cul4B has been observed using overexpression vectors but not on endogenous samples.
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经测试应用
适用于: WB, IP, IHC-Pmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human Cullin 4A/CUL-4A aa 700-800 (C terminal). The exact sequence is proprietary.
Database link: Q13619 -
阳性对照
- WB: HeLa, A375, 293T, HEK-293 transfected with human full length Cul4A and Cul4B expression vectors whole cell lysates. IHC-P: Human breast carcinoma, Hepatocellular carcinoma, Colonic adenocarcinoma, Ovarian carcinoma tissue. IP: HeLa.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR3198 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab92554于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/20000 - 1/100000. Predicted molecular weight: 88 kDa.
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IP |
1/50.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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说明 |
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WB
1/20000 - 1/100000. Predicted molecular weight: 88 kDa. |
IP
1/50. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
靶标
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功能
Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. In association with RBX1, DDB1 and DDB2 is required for histone H3 and histone H4 ubiquitination in response to ultraviolet and may be important for subsequent DNA repair. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip. Is involved in ubiquitination of HOXA9. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Belongs to the cullin family. -
翻译后修饰
Neddylated. Deneddylated via its interaction with the COP9 signalosome (CSN) complex (By similarity). Deneddylated by Epstein-Barr virus BPLF1 leading to a S-phase-like environment that is required for efficient replication of the viral genome. - Information by UniProt
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数据库链接
- Entrez Gene: 8451 Human
- Omim: 603137 Human
- SwissProt: Q13619 Human
- Unigene: 339735 Human
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别名
- 2810470J21Rik antibody
- AW495282 antibody
- CUL 4A antibody
see all
图片
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All lanes : Anti-Cullin 4A/CUL-4A antibody [EPR3198] (ab92554) at 1/10000 dilution
Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) transfected with blank vectors whole cell lysate
Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) transfected with human full length Cul4B expression vectors whole cell lysate
Lane 3 : HEK-293 (Human embryonic kidney epithelial cell) transfected with human full length Cul4A expression vectors whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 88 kDa
Observed band size: 104,88 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Ab92554 recognizes human Cul4A and Cul4B overexpression vectors.
ab180602 was used as a loading control.
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All lanes : Anti-Cullin 4A/CUL-4A antibody [EPR3198] (ab92554) at 1/20000 dilution
Lane 1 : Hela cell lysate
Lane 2 : A375 cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 88 kDa -
ab92554 at 1/100 dilution staining Cullin 4A/CUL-4A in Human breast carcinoma by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ab92554 showing positive staining in Hepatocellular carcinoma tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ab92554 showing positive staining in Colonic adenocarcinoma tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ab92554 showing positive staining in Ovarian carcinoma tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Cullin 4A/CUL-4A was immunoprecipitated from 0.35 mg HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg with 92554 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg
Lane 2: ab92554 IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab92554 in HeLa whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (17)
ab92554 被引用在 17 文献中.
- Ishida K et al. Glymphatic system clears extracellular tau and protects from tau aggregation and neurodegeneration. J Exp Med 219:N/A (2022). PubMed: 35212707
- Jones TM et al. Targeted CUL4A inhibition synergizes with cisplatin to yield long-term survival in models of head and neck squamous cell carcinoma through a DDB2-mediated mechanism. Cell Death Dis 13:350 (2022). PubMed: 35428778
- Li X et al. Lentivirus-mediated short hairpin RNA interference of CENPK inhibits growth of colorectal cancer cells with overexpression of Cullin 4A. World J Gastroenterol 28:5420-5443 (2022). PubMed: 36312839
- Weng SW et al. Autophagy Plays a Role in the CUL4A-Related Poor Prognosis of Intrahepatic Cholangiocarcinoma. Pathol Oncol Res 27:602714 (2021). PubMed: 34257560
- Liu X et al. Upregulated lncRNA DLX6-AS1 underpins hepatocellular carcinoma progression via the miR-513c/Cul4A/ANXA10 axis. Cancer Gene Ther 28:486-501 (2021). PubMed: 33277615