小鼠单克隆抗体[mAbcam60626] to Cripto1
Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human Cripto1.
(Peptide available as ab23310.)
- This antibody gave a positive signal in Cripto1 (Human) Recombinant Protein.
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact firstname.lastname@example.org or you can find further information here.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4
Concentration information loading...
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 10 µg/ml. Detects a band of approximately 21 kDa using a Cripto1 Recombinant Protein (predicted molecular weight: 16-20 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.
Preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts.
Contains 1 EGF-like domain.
Information by UniProt
- CR antibody
- CRGF antibody
- Cripto 1 antibody
Western blot - Anti-Cripto1 antibody [mAbcam60626] (ab60626)
Anti-Cripto1 antibody [mAbcam60626] (ab60626) at 10 µg/ml + Cripto1 Recombinant Protein at 0.1 µgSecondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.Predicted band size:
16-20 kDaObserved band size:
21 kDa (why is the actual band size different from the predicted?
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"