重组Anti-Cripto1/CRIPTO抗体[EPNCIR106A] (ab108391)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPNCIR106A] to Cripto1/CRIPTO
- Suitable for: ICC/IF, Flow Cyt (Intra), WB, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Cripto1/CRIPTO抗体[EPNCIR106A]
参阅全部 Cripto1/CRIPTO 一抗 -
描述
兔单克隆抗体[EPNCIR106A] to Cripto1/CRIPTO -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, Flow Cyt (Intra), WB, IPmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab84062) -
阳性对照
- WB and IP: NCCIT cell lysate. Flow Cyt (intra) and ICC/IF: NCCIT cells.
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常规说明
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of David Solomon. View antibodies from NCI Center for Cancer Research Collaboration.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Allow to warm to room temp and agitate gently before aliquotting. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPNCIR106A -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Conjugation kits
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108391于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/500.
For unpurified use at 1/100 -1/250. |
|
Flow Cyt (Intra) |
Use at an assay dependent concentration.
|
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WB |
1/1000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).Can be blocked with Recombinant human Cripto1/CRIPTO protein (Fc Chimera) (ab84062).
|
|
IP |
1/10 - 1/100.
|
说明 |
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ICC/IF
1/500. For unpurified use at 1/100 -1/250. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
WB
1/1000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).Can be blocked with Recombinant human Cripto1/CRIPTO protein (Fc Chimera) (ab84062). |
IP
1/10 - 1/100. |
靶标
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功能
Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm. -
组织特异性
Preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts. -
序列相似性
Contains 1 EGF-like domain. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 6997 Human
- Omim: 187395 Human
- SwissProt: P13385 Human
- Unigene: 385870 Human
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别名
- CR antibody
- CRGF antibody
- Cripto 1 antibody
see all
图片
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Intracellular Flow Cytometry analysis of NCCIT (human pluripotent embryonal carcinoma) cells labeling Cripto1/CRIPTO with purified ab108391at 1/2200 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (ab108391) at 1/2000 dilution (purified) + NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 21 kDaBlocking and diluting buffer: 5% NFDM/TBST
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ab108391 (purified) at 1:30 dilution (2ug) immunoprecipitating Cripto1/CRIPTO in NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate.
Lane 1 (input): NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate 10ug
Lane 2 (+): ab108391 & NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108391 in NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution. -
Immunocytochemistry/ Immunofluorescence analysis of NCCIT (Human pluripotent embryonic carcinoma epithelial cell) cells labeling Cripto1/CRIPTO with Purified ab108391 at 1:500 dilution (1.1μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (ab108391) at 1/1000 dilution (unpurified) + NCCIT cell lysate at 10 µg
Predicted band size: 21 kDa
Observed band size: 21 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab108391 被引用在 6 文献中.
- Li H et al. Defining the Pluripotent Marker Genes for Identification of Teleost Fish Cell Pluripotency During Reprogramming. Front Genet 13:819682 (2022). PubMed: 35222539
- Du T et al. MiR-138-1-3p alters the stemness and radiosensitivity of tumor cells by targeting CRIPTO and the JAK2/STAT3 pathway in nasopharyngeal carcinoma. Ann Transl Med 9:485 (2021). PubMed: 33850882
- Hu C et al. Exosomal Cripto-1 Serves as a Potential Biomarker for Perihilar Cholangiocarcinoma. Front Oncol 11:730615 (2021). PubMed: 34434900
- Gudbergsson JM & Duroux M An evaluation of different Cripto-1 antibodies and their variable results. J Cell Biochem N/A:N/A (2019). PubMed: 31310365
- Lo RC et al. Cripto-1 contributes to stemness in hepatocellular carcinoma by stabilizing Dishevelled-3 and activating Wnt/ß-catenin pathway. Cell Death Differ 25:1426-1441 (2018). PubMed: 29445127
- Aykul S et al. Biochemical and Cellular Analysis Reveals Ligand Binding Specificities, a Molecular Basis for Ligand Recognition, and Membrane Association-dependent Activities of Cripto-1 and Cryptic. J Biol Chem 292:4138-4151 (2017). PubMed: 28126904