Application
Western blot
Sample
Mouse Tissue lysate - other (muscle)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
30 µg
Specification
muscle
Blocking step
Blocking buffer for fluorescent western blocking as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/400
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: IR DYE donkey anti-mouse
Host species: Donkey
Clonality: Polyclonal
Conjugation: Other
Host species: Donkey
Clonality: Polyclonal
Conjugation: Other
Detection
Detection method
Odyssey
Exposure
4 minute(s) and 0 second(s)
Bands
Specific: 45 kDa Non-specific: 90, 39 kDa
Additional data
Abcam response
Thank you for testing ab78408 in an untested species, mouse in WB. We welcome any other feedback for mouse and we invite you to participate in our AbTrial program http://www.abcam.com/content/abtrial-program-test-risk-free.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MRS. Nathalie Da Silva
Verified customer
提交于 Jul 13 2016