Complex IV Rodent Enzyme Activity Microplate Assay试剂盒(ab109911)


  • 产品名称
    Complex IV Rodent Enzyme Activity Microplate Assay试剂盒
    参阅全部 Complex IV 试剂盒
  • 检测方法
  • 样品类型
    Cell culture extracts, Tissue
  • 检测类型
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Mouse, Rat
  • 产品概述

    Complex IV Rodent Enzyme Activity Microplate Assay Kit (ab109911) is used to determine the activity of cytochrome c oxidase in a mouse sample with speed and simplicity. The COX enzyme is immunocaptured within the wells of the microplate and activity is determined colorimetrically by following the oxidation of reduced cytochrome c by the absorbance change at 550 nm. Included in this kit for performance of the activity assay are buffer, detergent, substrate, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.



  • 说明

    Buffer, detergent, and microplate should be stored at 4°C, Reagent C (reduced cytochrome c) should be stored at -20°C.

  • 经测试应用
    适用于: Functional Studiesmore details
  • 平台



Our Abpromise guarantee covers the use of ab109911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.


  • Abcam's enzyme activity assays apply a novel approach, whereby target enzymes are first immunocaptured from tissue or cell samples before subsequent functional analysis. All of our ELISA kits utilize highly validated monoclonal antibodies and proprietary buffers, which are able to capture even very large enzyme complexes in their fully-intact, functionally-active states. Capture antibodies are pre-coated in the wells of premium Nunc MaxiSorp™ modular microplates, which can be broken into 8-well strips. After the target has been immobilized in the well, substrate is added, and enzyme activity is analyzed by measuring the change in absorbance of either the substrate or the product of the reaction (depending upon which enzyme is being analyzed). By analyzing the enzyme's activity in an isolated context, outside of the cell and free from any other variables, an accurate measurement of the enzyme's functional state can be understood.
  • Figure 1. To determine the activity in the sample, calculate the slope by using microplate software or by manual calculations using one of the two methods shown. Compare the sample rate with the rate of the control (normal) sample and with the rate of the null (background) to get the relative Complex IV activity. (A)The rate is determined by calculating the gradient of the initial slope over the linear region. (B) The rate is determined by calculating the slope between two points within the linear region.



This product has been referenced in:
  • Zhang K & Jiang D RhoA inhibits the hypoxia-induced apoptosis and mitochondrial dysfunction in chondrocytes via positively regulating the CREB phosphorylation. Biosci Rep 37: (2017). Read more (PubMed: 28254846) »
  • Dadson K  et al. The E3 ligase Mule protects the heart against oxidative stress and mitochondrial dysfunction through Myc-dependent inactivation of Pgc-1a and Pink1. Sci Rep 7:41490 (2017). Read more (PubMed: 28148912) »

See all 29 Publications for this product


I used isolated protein from guinea pig placenta to perform the experiment with this kit. There is no activity detected.

Abcam user community

Verified customer

提交于 Feb 07 2017

1) Yes, the mitochondrial isoaltion kit is suitable for use with all kits listed.

2) Suspend the prepared mitochondria to 5.5 mg/mL as described in the attached document and follow from step 5 (adding detergent to mitochondia) to generat...

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The protocol makes specific reference to mitochondria and tissue extracts. When it comes to cells, there is a significant loss of protein in the pellet after extraction as you mentioned on the phone. I would recommend either re-measuring the concentr...

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We provide the cytochrome c in reduced form for this kit.
I can say the protein is active and also the detergent is mild. So if cytochrome c functions as a dimer then you can anticipate this conformation in the assay solution.
We are unsure wh...

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30ug Lung mitochondrion samples from mice in buffer containing 250 mM sucrose, 20 mM HEPES, 1 mM EDTA, 1 mM EGTA, 0.25% protease inhibitor, and 0.5% BSA, pH 7.4 were used for the assay.

Dr. Amit Agarwal

Verified customer

提交于 Jul 24 2013

Thank you for your technical enquiry and interest in our products.

I have discussed this with my colleagues in the Lab. This kit (ab109911: Complex IV Rodent Enzyme) measures in a standard spectrophotometer the absorbance of reduced cytochrom...

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Thank you for your interest.

The product code number for this item is ab140219. However, it is not published so the datasheet can't get accessed on the public site.

I would advise to place an order via e-mail:

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Thank you for contacting us and your interest in our products. I am sorry for the delay in getting back to you.

The three kits mentioned (ab109721, ab109911 and ab109908) use 10% lauryl maltoside detergent to prepare the sample at 5 mg/mL. The...

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Thank you for your contact.

I have discussed your enquiry with my colleagues and can confirm that Reagent C is available for special request. The sale price is USD50.00 or GBP40.00 /unit. We currently have this item in stock.

Could ...

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Thank you for your enquiry.

I can confirm that In each of these kits we use a non-ionic native detergent at an optimized concentration to maintain OXPHOS complex assembly and activity. This detergent is 1% final N-dodecyl-b-D-maltoside ...

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