Application
Western blot
Sample
Hamster Cell lysate - whole cell (BHK-21 (Kidney Fibroblasts))
Gel Running Conditions
Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)
Loading amount
50000 cells
Specification
BHK-21 (Kidney Fibroblasts)
Blocking step
Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Li-Cor Odyssey Blocking Buffer (TBS)
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: IRDye 800CW Goat anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
Near-Infrared Fluorescence Imaging (NIR)
Exposure
4 minute(s) and 30 second(s)
Bands
Specific: 210 kDa Non-specific: 17, 90 kDa
Additional data
Additional Notes
Both anti-Collagen IV and anti-beta actin (ab6276) antibodies were used for the western blot. The anti-Collagen IV antibody itself detected a specific 210 kDa band (green band) and some non-specific bands at 17 & 90. The 42 kDa red band is the beta actin band (red band).
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提交于 Jul 22 2016