Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (femur (embryonic))
Permeabilization
Yes - 40%OCT
Specification
femur (embryonic)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1xTBS with 1% BSA
Dilution
1/100
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Donkey anti-Rabbit IgG (H+L) Alexa Fluor® 546
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 546
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 546
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jun 22 2015