Application
Western blot
Sample
Human Cell lysate - whole cell (epithelial)
Gel Running Conditions
Reduced Denaturing (6% Tris-Glycine)
Loading amount
10 µg
Specification
epithelial
Blocking step
Licor TBS blocking buffer as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/5000
Secondary antibody
Dilution
1/15000
Name
Non-Abcam antibody was used: IRDye® 800CW Goat anti-Rabbit IgG (H + L)
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
IR
Exposure
1 second(s)
Bands
Specific: 150 kDa
Positive control
recomb human Col I
Additional data
Additional Notes
lane 1: markers
lane 2: rhCol I
lanes 3 & 4: total cell lysate from hu epithelial cancer cell line
2 bands = a1 and a2 chains
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Aug 30 2016