Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse Bone)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization
Yes - PBS with 3% Triton
Specification
Mouse Bone
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS with 0.025% Triton
Dilution
1/500
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Goat Anti Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
Antigen Retrival: Sodium Citrate 10mM. 95C heat for 10min.
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提交于 Jul 27 2015