Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Mouse Cell (colon tumor)
Specification
colon tumor
Permeabilization
Yes - Boiling in citrate buffer
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA/PBS
Dilution
1/250
Secondary antibody
Name
Abcam antibody:Goat anti-Rabbit IgG H&L (DyLight 594) secondary antibody
Dilution
1/1000
Additional data
Additional Notes
collagen 1 costained with ecadherin ab53033, excellent staining!
Abcam response
Thank you very much for sharing this beautiful image. Any feedback provided by our customers is much appreciated.
For users wishing to test our products in untested species or applications, we would encourage them to participate in our Abtrial program, by which you will beneficiate from a discount off your next purchase for the value of the product tested by submitting your results to our website.
The Terms and Conditions of this offer can be found at: www.abcam.com/abtrial.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Charles Pallangyo
Verified customer
提交于 Jun 25 2013