Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
Permeabilization
No
Specification
Skin
Blocking step
Casein as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
Other product details
Incubation time
30 minute(s) · Diluent: 1% BSA in TBS
Concentration
10 µg/ml
Additional data
Additional Notes
Followed up anti-mouse secondary with Envision Rabbit from Dako. Ab does not react with rat collagen. Ab works very well on human tissue using the same protocol.
Abcam response
Thank you so much for testing this antibody in rat. We appreciate very much your efforts, but are sorry to hear that the antibody did not work well in this species.
The customer received a discount on a future antibody purchase for testing this product. We would welcome additional feedback from other customers as well.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Mar 15 2016