Anti-Coilin抗体[Pdelta] (ab11822)
Key features and details
- Mouse monoclonal [Pdelta] to Coilin
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG1
概述
-
产品名称
Anti-Coilin抗体[Pdelta]
参阅全部 Coilin 一抗 -
描述
小鼠单克隆抗体[Pdelta] to Coilin -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human Coilin (C terminal).
-
表位
Monoclonal Anti-coilin reacts with an epitope located between amino acids 363 and 481 of human coilin. -
阳性对照
- HeLa nuclear extract.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
Pdelta -
同种型
IgG1 -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab11822于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use at an assay dependent concentration.
|
|
ICC/IF | (1) |
Use a concentration of 1 µg/ml.
|
说明 |
---|
WB
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 1 µg/ml. |
靶标
-
功能
Is a component of the nuclear coiled bodies (CBS) which are involved in the function or assembly/disassembly of nucleoplasmic snRNPs. During mitosis, CBS disassemble, coinciding with a mitotic-specific phosphorylation of p80 coilin. -
组织特异性
Found in all the cell types examined. -
序列相似性
Belongs to the coilin family. -
细胞定位
Nucleus. Nuclear coiled body located in the interchromatin space between the nucleolus and the nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 8161 Human
- Entrez Gene: 12812 Mouse
- Omim: 600272 Human
- SwissProt: P38432 Human
- SwissProt: Q5SU73 Mouse
- Unigene: 532795 Human
-
别名
- CLN 80 antibody
- CLN80 antibody
- COIL antibody
see all
图片
-
ICC/IF image of ab11822 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11822, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
数据表及文件
-
SDS download
-
Datasheet download
文献 (39)
ab11822 被引用在 39 文献中.
- Binda O et al. SMA-linked SMN mutants prevent phase separation properties and SMN interactions with FMRP family members. Life Sci Alliance 6:N/A (2023). PubMed: 36375840
- Suzuki H et al. The 3' Pol II pausing at replication-dependent histone genes is regulated by Mediator through Cajal bodies' association with histone locus bodies. Nat Commun 13:2905 (2022). PubMed: 35614107
- Luessing J et al. A function for ataxia telangiectasia and Rad3-related (ATR) kinase in cytokinetic abscission. iScience 25:104536 (2022). PubMed: 35754741
- Chen L et al. Loss of Human TGS1 Hypermethylase Promotes Increased Telomerase RNA and Telomere Elongation. Cell Rep 30:1358-1372.e5 (2020). PubMed: 32023455
- Takahashi H et al. The role of Mediator and Little Elongation Complex in transcription termination. Nat Commun 11:1063 (2020). PubMed: 32102997