Application
Western blot
Sample
Clostridium botulinum Purified protein (cell lysate)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
5 µg
Specification
cell lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: TBS
Secondary antibody
Name
Abcam antibody:Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase)
Detection
Detection method
BCIP
Exposure
20 minute(s) and 0 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. chung sei kim
Verified customer
提交于 Jun 20 2012