重组Anti-Cleaved NEAS (Asp1185)抗体[EPR3830-17] (ab92373)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3830-17] to Cleaved NEAS (Asp1185)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Cleaved NEAS (Asp1185)抗体[EPR3830-17]
参阅全部 Cleaved NEAS (Asp1185) 一抗 -
描述
兔单克隆抗体[EPR3830-17] to Cleaved NEAS (Asp1185) -
宿主
Rabbit -
特异性
Only detects the large fragment of Alpha Fodrin resulting from cleavage at aspartic acid 1185. -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human Cleaved NEAS (Asp1185) aa 1150-1250. The exact sequence is proprietary.
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阳性对照
- HeLa + Camptothecin cell lysate, HeLa cell lysate, Jurkat + Staurosporine cell lysate, Jurkat cell lysate
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3830-17 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab92373于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/10000 - 1/50000. Predicted molecular weight: 285 kDa.
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说明 |
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WB
1/10000 - 1/50000. Predicted molecular weight: 285 kDa. |
靶标
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功能
Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane. -
疾病相关
Epileptic encephalopathy, early infantile, 5 -
序列相似性
Belongs to the spectrin family.
Contains 3 EF-hand domains.
Contains 1 SH3 domain.
Contains 23 spectrin repeats. -
翻译后修饰
Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro. -
细胞定位
Cytoplasm, cytoskeleton. Cytoplasm, cell cortex. Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells. - Information by UniProt
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数据库链接
- Entrez Gene: 6709 Human
- SwissProt: Q13813 Human
- Unigene: 372331 Human
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别名
- Alpha-II spectrin antibody
- Fodrin alpha chain antibody
- non-erythrocytic 1 antibody
see all
图片
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All lanes : Anti-Cleaved NEAS (Asp1185) antibody [EPR3830-17] (ab92373)
Lane 1 : HeLa + Camptothecin cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat + Staurosporine cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 285 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab92373 尚未被引用在任何文献中。