Application
Western blot
Sample
Human Cell lysate - whole cell (epithelial ovacar cell line)
Gel Running Conditions
Reduced Denaturing (7.5%)
Loading amount
100 µg
Treatment
0-200uM Perifosine
Specification
epithelial ovacar cell line
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
4 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 2% MILK
Dilution
1/1000
Secondary antibody
Dilution
1/20000
Name
Non-Abcam antibody was used: Sigma Anti-Mouse
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Pierce ECL Western Blotting Substrate
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 116 kDa Non-specific: 89, 70 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jul 26 2016