重组Anti-Cleaved PARP1抗体[E51] - BSA and Azide free (ab203467)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E51] to Cleaved PARP1 - BSA and Azide free
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Cleaved PARP1抗体[E51] - BSA and Azide free
参阅全部 Cleaved PARP1 一抗 -
描述
兔单克隆抗体[E51] to Cleaved PARP1 - BSA and Azide free -
宿主
Rabbit -
特异性
This antibody is specific for the p25 cleaved form of human PARP1.
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经测试应用
适用于: WB, IHC-Pmore details
不适用于: ICC/IF -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Chinese hamster -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Jurkat whole cell lysate (ab7899). HeLa and RAW 264.7 whole cell lysate. HAP1, HeLa, NIH/3T3 and PC-12 treated with 1uM Staurosporine. Jukat cells treated with camptothecin. Jukat cells treated with 15-Acetoxyscirpenol. IHC-P: Rat colon tissue. Human ovarian cancer and breast carcinoma tissue.
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常规说明
ab203467 is the carrier-free version of ab32064.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.20
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
E51 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab203467于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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说明 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites. -
序列相似性
Contains 1 BRCT domain.
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
Contains 2 PARP-type zinc fingers. -
翻译后修饰
Phosphorylated by PRKDC and TXK.
Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
S-nitrosylated, leading to inhibit transcription regulation activity. -
细胞定位
Nucleus. Nucleus, nucleolus. Localizes at sites of DNA damage. - Information by UniProt
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数据库链接
- Entrez Gene: 100689463 Chinese hamster
- Entrez Gene: 142 Human
- Entrez Gene: 11545 Mouse
- Entrez Gene: 25591 Rat
- Omim: 173870 Human
- SwissProt: Q9R152 Chinese hamster
- SwissProt: P09874 Human
- SwissProt: P11103 Mouse
see all -
别名
- ADP ribosyltransferase diphtheria toxin like 1 antibody
- ADP ribosyltransferase NAD(+) antibody
- ADP-ribosyltransferase diphtheria toxin-like 1 antibody
see all
图片
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All lanes : Anti-Cleaved PARP1 antibody [E51] (ab32064) at 1/10000 dilution
Lane 1 : Wild-type A549 control staurosporine (0 uM, 72 h) cell lysate
Lane 2 : Wild-type A549 treated staurosporine (3 uM, 24 h) cell lysate
Lane 3 : Wild-type A549 control staurosporine (3 uM, 72 h) cell lysate
Lane 4 : PARP1 knockout A549 treated staurosporine (3 uM, 24 h) cell lysate
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab32064).
Western blot: Anti-PARP1 antibody [E51] (ab32064) staining at 1/10000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab32064 was shown to bind specifically to PARP1. A band was observed at 27 kDa in wild-type A549 cell lysates with no signal observed at this size in PARP1 knockout cell line. To generate this image, wild-type and PARP1 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution. -
All lanes : Anti-Cleaved PARP1 antibody [E51] (ab32064) at 1/10000 dilution
Lane 1 : Wild-type (1uM Staurosporine for 3hrs) HAP1 cell lysate
Lane 2 : Wild-type (Staurosporine control) HAP1 cell lysate
Lane 3 : PARP1 knockout (1uM Staurosporine for 3hrs) HAP1 cell lysate
Lane 4 : PARP1 knockout (Staurosporine control) HAP1 cell lysate
Lane 5 : HeLa (1uM Staurosporine for 3hrs) cell lysate
Lane 6 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab32064).
Lanes 1 - 6: Merged signal (red and green). Green - ab32064 observed at 27 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.
ab32064 was shown to react with Cleaved PARP1 in wild-type HAP1 cells in Western blot with loss of signal observed in PARP1 knockout sample.Wild-type HAP1 and PARP1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab32064 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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Immunohistochemical staining of paraffin embedded rat colon with purified ab32064 at a working dilution of 1/100. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counterstained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
PBS was used instead of the primary antibody as the negative control (inset).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32064).
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Immunohistochemical staining of paraffin embedded human ovarian carcinoma with purified ab32064 at a working dilution of 1 in 100. The secondary antibody used is a HRP polymer for rabbit IgG. Counterstained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
PBS was used instead of the primary antibody as the negative control (inset).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32064).
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (2)
ab203467 被引用在 2 文献中.
- Ji G et al. Melatonin inhibits proliferation and viability and promotes apoptosis in colorectal cancer cells via upregulation of the microRNA-34a/449a cluster. Mol Med Rep 23:N/A (2021). PubMed: 33398374
- Pan Y et al. Baicalin prevents the apoptosis of endplate chondrocytes by inhibiting the oxidative stress induced by H2O2. Mol Med Rep 16:2985-2991 (2017). PubMed: 28677799